A New Fluorescent Method to Detect Sulfamidase Activity in Blood, Tissue Extracts and Dried Blood Spots
Abstract Mucopolysaccharidosis type IIIA (MPS IIIA) is a lysosomal storage disorder due to the deficient activity of sulfamidase (SGSH).Traditionally, measurement of this Chef Hats enzymatic activity has been performed using a fluorescently (4-MU) labeled glycoside substrate.While this substrate is inexpensive and readily available, the current met